MDx Beginner Guide

Molecular Diagnostics (MDx)

Definition

  • Molecular diagnostics (MDx) is a collection of techniques used for diagnosing diseases or pathogens.

Features of MDx

  • Provide sensitive and accurate results
  • Reduce the test time with high throughput
  • Can be applied for diagnosing various diseases

Essential process of MDx using Real-Time PCR

  • Nucleic acid extraction
  • Nucleic acid amplification (Real-Time PCR)
  • Nucleic Acid Extraction

    This step is for purifying DNA/RNA from samples. The performance of the extraction is determined by the purity and the yield of the targeted gene.

  • Real-Time PCR

    The sensitivity and specificity of the test are determined by the amplification of the target gene.